Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
Ekaterinburg, Ekaterinburg, Russian Federation
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
employee
Ekaterinburg, Ekaterinburg, Russian Federation
Subject. Microbial landscape of the oral cavity is a highly sensitive systemic indicator reacting to changes in different body organs and systems by qualitative and quantitative shifts. The unique ability of microbiocenosis of the oral cavity to self-regulate and maintain the ecological balance creates a personal health profile of an individual and assumes that a timely correction of microbiocenosis shall be added to required treatment patterns for various dental and somatic diseases. This approach ensures the protective effect of growth of opportunistic pathogenic bacteria improving quality of therapy and patient recovery time. Objectives. To study dynamics of clinical and microbiological changes in the oral cavity in patients with periodontitis and hypothyroidism on the background of complex therapy with inclusion of synbiotic Maxilac. Materials and methods. Based in the Ural State Medical University Department of Therapeutic Dentistry and Propedeutics of Dental Decease, the study of oral microbiology was carried out on 50 generalized moderate periodontitis patients aged 44 to 59 years during the period from 2017 to 2021. The average age of the subjects was 50.50±3.5 years. Two groups were formed: the main group (30 patients with “primary hypothyroidism” receiving hormone therapy) and the comparison group (20 patients). The patients in the main group were prescribed general therapy by the endocrinologist based on the phase, stage and the severity of the disease. During examination all patients underwent the complex clinical and laboratory evaluation before treatment, in its course and after it, as well they were subjected to full mouth debridement and the course of the complex periodontal therapy in accordance with clinical recommendations. Microbiota was corrected by active ingredient probiotic (lyophilized probiotic bacteria) — 4.5*109 CFU (colony-forming unit): Lactobacillus elveticus, lactis, rhamnosus, plantarum, casei; Bifidobacterium longum, breve, bifidum; Sreptococcus thermophilus. Results. The study of microbiocenosis of the oral cavity of patients with primary hypothyroidism demonstrated changes in species and the number of microorganisms in the oral cavity. Conclusions. Timely diagnostics and correction of disbiotic changes included in the dental treatment protocol will allow to select the most appropriate dental treatment strategy.
oral microbiota, microbiological evaluation, hypothyroidism, synbiotic, normobiom, periodontitis
1. Andreeva I.V., Stecyuk O.U. Novyy probioticheskiy shtamm Streptococcus salivarius K12 v klinicheskoy praktike. KMAH. 2019;21;2:92-99. [I.V. Andreeva, O.U. Stetsyuk. A new probiotic strain Streptococcus salivarius K12 in clinical practice. KMAKH. 2019;21;2:92-99. (In Russ.)].
2. Bagdasaryan N.P., Erichev V.V., Ovcharenko E.S., Aksenova T.V., Ermoshenko L.S., Karapetov S.A. Korrekciya citokinovogo balansa i mikrobiocenoza polosti rta pacientov s hronicheskimi vospalitel'nymi zabolevaniyami parodonta pri pomoschi probiotikov. Mediko-farmacevticheskiy zhurnal «Pul's». 2019;10:42-52. [N.P. Bagdasaryan, V.V. Yerichev, E.S. Ovcharenko, T.V. Aksenova, L.S. Yermoshenko, S.A. Karapetov. Correction of the cytokine balance and microbiocenosis of the oral cavity in patients with chronic inflammatory periodontal diseases using probiotics. Medical and pharmaceutical journal "Pulse". 2019;10:42-52. (In Russ.)].
3. Biktemerova O.O., Redinova T.L. Dinamika klinicheskih, immunologicheskih i mikrobiologicheskih pokazateley polosti rta u pacientov s hronicheskim generalizovannym parodontitom legkoy i sredney stepeni tyazhesti pri lechenii probiotikami. Parodontologiya. 2016;2(79):10-15. [O.O. Biktemerova, T.L. Redinova. Dynamics of clinical, immunological and microbiological parameters of the oral cavity in patients with chronic generalized periodontitis of mild and moderate severity during treatment with probiotics. Periodontology. 2016;2(79):10-15. (In Russ.)].
4. Veysgeym L.D., Gavrikova L.M., Dubacheva S.M. Sovremennye problemy diagnostiki i lecheniya kandidoza polosti rta. DENTAL MAGAZINE. 2016;11(155):32-34. [L.D. Veisgeym, L.M. Gavrikova, S.M. Dubacheva. Modern problems of diagnosis and treatment of oral candidiasis. DENTAL MAGAZINE. 2016;11(155):32-34. (In Russ.)].
5. Gaysina E. F., Elovikova T. M., Izmozherova N. V., Motousova S. A. Antibiotikoprofilaktika Infekcionnogo Endokardita Na Stomatologicheskom Prieme. Problemy stomatologii. 2019;15;2:4-10. [E.F. Gaisina, T.M. Elovikova, N.V. Izmozherova, S.A. Motousova. Antibiotic prophylaxis of Infectious Endocarditis at the Dental Appointment. Actual problems in dentistry. 2019;15;2:4-10. (In Russ.)]. https://www.elibrary.ru/item.asp?id=39165955
6. Gorelov A.V., Kanner E.V., Maksimov M.L., Ermolaeva A.S., Voznesenskaya A.A., Dadasheva K.N. Lactobacillus Rhamnosus Gg: Klinicheskie aspekty primeneniya s pozicii dokazatel'noy mediciny. Medicinskiy Sovet. 2018;17:66-73. [A.V. Gorelov, E.V. Kanner, M.L. Maksimov, A.S. Ermolaeva, A.A. Voznesenskaya, K.N. Dadasheva. Lactobacillus Rhamnosus Gg: Clinical aspects of application from the perspective of evidence-based medicine. Medical Council. 2018;17:66-73. (In Russ.)]. https://www.elibrary.ru/item.asp?id=36289773
7. Dzyuba E.V., Nagaeva M.O., Zhdanova E.V. Rol' immunologicheskih processov v razvitii vospalitel'nyh processov paradonta i vozmozhnosti ih korrekcii. Problemy stomatologii. 2019;15;2:25-31. [E.V. Dzyuba, M.O. Nagaeva, E.V. Zhdanova. The role of immunological processes in the development of inflammatory processes of the periodontal and the possibility of their correction. Actual problems in dentistry. 2019;15;2:25-31. (In Russ.)]. https://www.elibrary.ru/item.asp?id=39165958
8. Elovikova T.M., Ermishina E.Yu., Sablina S.N., Grigor'ev S.S., Koscheev A.S. Kliniko-laboratornoe issledovanie fiziko-himicheskih svoystv novoy zubnoy pasty s kompleksom Hyaluron-Ti Forte. Problemy stomatologii. 2020;16;4:46-50. [T.M. Elovikova, E.Yu. Ermishina, S.N. Sablina, S.S. Grigoriev, A.S. Koshcheev. Clinical and laboratory study of the physical and chemical properties of a new toothpaste with the Hyaluron-Ti Forte complex. Actual problems in dentistry. 2020;16;4:46-50. (In Russ.)]. https://www.elibrary.ru/item.asp?id=44667776
9. Elovikova T.M., Karaseva V.V., Zholudev S.E., Skurihina Ya.S., Mirsaev T.D. Harakteristika parametrov gomeostaticheskih mehanizmov polosti rta u molodyh kuril'schikov tabaka. Ural'skiy medicinskiy zhurnal. 2020;9(192):44-48. [T.M. Elovikova, V.V. Karaseva, S.E. Zholudev, Ya.S. Skurikhina, T.D. Mirsaev. Characterization of the parameters of the homeostatic mechanisms of the oral cavity in young tobacco smokers. Ural Medical Journal. 2020;9(192):44-48. (In Russ.)]. https://www.elibrary.ru/item.asp?id=44206402
10. Elovikova T.M., Sablina S.N., Koscheev A.S. Gigienicheskie aspekty resheniya problemy galitoza u molodyh kuril'schikov tabaka. Aktual'nye Voprosy Stomatologii : sbornik nauchnyh trudov, posvyaschennyy osnovatelyu kafedry ortopedicheskoy stomatologii KGMU professoru Isaaku Mihaylovichu Oksmanu. Kazan'. 2021:173-177. [T.M. Elovikova, S.N. Sablina, A.S. Koshcheev. Hygienic aspects of solving the problem of halitosis in young tobacco smokers. Actual Issues of Dentistry: a collection of scientific papers dedicated to the founder of the Department of Prosthetic Dentistry of KSMU, Professor Isaak Mikhailovich Oksman. Kazan. 2021:173-177. (In Russ.)].
11. Zaharova I.N. Shtammospecifichnost' probiotikov. Effektivnaya farmakoterapiya. 2017;11:26-29. [I.N. Zakharova. Strain-specificity of probiotics. Effective pharmacotherapy. 2017;11:26-29. (In Russ.)].
12. Karakov K.G., Vlasova T.N., Oganyan A.V., Hachaturyan A.E., Timircheva V.V., Aslamova K.E., Solov'eva V.S. Kriterii vybora metoda korrekcii disbakterioza organov polosti rta. Problemy stomatologii. 2020;16;2:17-21. [K.G. Karakov, T.N. Vlasova, A.V. Ohanyan, A.E. Khachaturyan, V.V. Timircheva, K.E. Aslamova, V.S. Solovieva. Criteria for choosing a method for correcting dysbacteriosis of the oral cavity organs. Actual problems in dentistry. 2020;16;2:17-21. (In Russ.)]. https://www.elibrary.ru/item.asp?id=43783702
13. Katola V.M., Tarasenko S.E., Komogorceva V.E. Vliyanie mikrobioty polosti rta na razvitie vospaleniya i somaticheskih zabolevaniy. Rossiyskiy stomatologicheskiy zhurnal. 2018;22(3):162-165. [V.M. Katola, S.E. Tarasenko, V.E. Komogortseva. The influence of the oral microbiota on the development of inflammation and somatic diseases. Russian Dental Journal. 2018;22(3):162-165. (In Russ.)]. https://www.elibrary.ru/item.asp?id=35419638
14. Klimova E.A., Sokolovich T.A., Brodina T.V. Mikrobiota polosti rta kak klyuch k ponimaniyu karioznogo processa. Vestnik SPbGU. Medicina. 2017;12;1:54-59. [E.A. Klimova, T.A. Sokolovich, T.V. Brodina. Oral cavity microbiota as a key to understanding the carious process. Bulletin of St. Petersburg State University. Medicine. 2017;12;1:54-59. (In Russ.)].
15. Kraynov S.V., Mihal'chenko V.F., Popova A.N., Yakovlev A.T., Linchenko I.V. Vliyanie immunomoduliruyuschey terapii na kletochnyy sostav desnevoy zhidkosti u lic pozhilogo vozrasta, stradayuschih parodontitom. Problemy stomatologii. 2018;14;1:21-25. [S.V. Krainov, V.F. Mikhalchenko, A.N. Popova, A.T. Yakovlev, I.V. Linchenko. The effect of immunomodulatory therapy on the cellular composition of the gingival fluid in the elderly with periodontitis. Actual problems in dentistry. 2018;14;1:21-25. (In Russ.)]. https://www.elibrary.ru/item.asp?id=32840684
16. Lukichev M.M., Ermolaeva L.A. Ispol'zovanie bakteriofagov i probiotikov v kompleksnom lechenii vospalitel'nyh zabolevaniy parodonta. Institut stomatologii. 2018;1:84-87. [M.M. Lukichev, L.A. Ermolaeva. The use of bacteriophages and probiotics in the complex treatment of inflammatory periodontal diseases. Institute of Dentistry. 2018;1:84-87. (In Russ.)]. https://www.elibrary.ru/item.asp?id=34964797
17. Mirsaeva F.Z., Gileva O.S., Hanov T.V., Kuznecova T.N., Baykova A.Yu., Garayshin R.M., Sharafutdinov A.N. Obosnovanie primeneniya probiotikov iz sporoobrazuyuschih bakteriy roda BACILLUS pri hronicheskom generalizovannom parodontite. Problemy stomatologii. 2020;16;2:59-63. [F.Z. Mirsaeva, O.S. Gileva, T.V. Khanov, T.N. Kuznetsova, A.Yu. Baykova, R.M. Garaishin, A.N. Sharafutdinov. Rationale for the use of probiotics from spore-forming bacteria of the genus BACILLUS in chronic generalized periodontitis. Actual problems in dentistry. 2020;16;2:59-63. (In Russ.)]. https://www.elibrary.ru/item.asp?id=43783708
18. Nazarov P.A. Al'ternativy antibiotikam: liticheskie fermenty bakteriofagov i fagovaya terapiya. Vestnik RGMU. 2018;1:5-15. [P.A. Nazarov. Alternatives to antibiotics: lytic enzymes of bacteriophages and phage therapy. Bulletin of the Russian State Medical University. 2018;1:5-15. (In Russ.)]. https://www.elibrary.ru/item.asp?id=35121826
19. Neschislyaev V.A., Mokin P.A., Fedorova T.V. K voprosu razrabotki vysokoeffektivnyh metabolitnyh probiotikov. Aktual'nye napravleniya nauchnyh issledovaniy: ot teorii k praktike. 2016;2-1(8):15-17. [V.A. Neschislyaev, P.A. Mokin, T.V. Fedorova. On the development of highly effective metabolic probiotics. Actual directions of scientific research: from theory to practice. 2016;2-1(8):15-17. (In Russ.)].
20. Ostrovskaya S.S. Mikrobiom rotovoy polosti i zdorov'e cheloveka. Vestnik problem biologii mediciny. 2018;1;2(143):35-38. [S.S. Ostrovskaya. Oral cavity microbiome and human health. Bulletin of problems of biology of medicine. 2018;1;2(143):35-38. (In Russ.)].
21. Romanenko I.G., Kekosh E.A. Vliyanie probiotika «Lineks forte» na oral'nyy disbioz u pacientov s hronicheskim generalizovannym parodontitom na fone autoimmunnogo tireoidita. Krymskiy terapevticheskiy zhurnal. 2018;4:57-60. [I.G. Romanenko, E.A. Kekosh. Influence of probiotic "Linex Forte" on oral dysbiosis in patients with chronic generalized periodontitis against the background of autoimmune thyroiditis. Crimean therapeutic journal. 2018;4:57-60. (In Russ.)]. https://www.elibrary.ru/item.asp?id=36807156
22. Sablina S.N., Elovikova T.M., Grigor'ev S.S., Epishova A.A., Bushueva E.Yu., Andreeva A.S., Shimova M.E., Sorokoumova D.V., Chagay A.A. Problema galitoza v kompleksnoy diagnostike i lechenii zabolevaniy parodonta. Literaturnyy obzor. Problemy stomatologii. 2021;17;1:32-43. [S.N. Sablina, T.M. Elovikova, S.S. Grigoriev, A.A. Epishova, E.Yu. Bushueva, A.S. Andreeva, M.E. Shimova, D.V. Sorokoumova, A.A.Chagai. The problem of halitosis in the complex diagnosis and treatment of periodontal diseases. Literature review. Actual problems in dentistry. 2021;17;1:32-43. (In Russ.)]. https://www.elibrary.ru/item.asp?id=45699791
23. Sablina S.N., Elovikova T.M., Grigor'ev S.S., Epishova A.A., Shimova M.E., Bushueva E.Yu. Sovremennyy vzglyad na ispol'zovanie transmukoidnyh terapevticheskih sistem v vide plenochnyh kompoziciy v parodontologii. Problemy stomatologii. 2020;16;3:11-17. [S.N. Sablina, T.M. Elovikova, S.S. Grigoriev, A.A. Epishova, M.E. Shimova, E.Yu. Bushueva. Modern view on the use of transmucoid therapeutic systems in the form of film compositions in periodontology. Actual problems in dentistry. 2020;16;3:11-17. (In Russ.)]. https://www.elibrary.ru/item.asp?id=44153717
24. Sablina S.N., Elovikova T.M., Grigor'ev S.S., Hromcova O.M., Zakroeva A.G., Epishova A.A., Karaseva V.V., Mirsaev T.D., Dorohina K.R. Rol' vitamina D v podderzhanii zdorov'ya polosti rta. Literaturnyy obzor. Problemy stomatologii. 2020;16;4:25-34. [S.N. Sablina, T.M. Elovikova, S.S. Grigoriev, O.M. Khromtsova, A.G. Zakroeva, A.A. Epishova, V.V. Karaseva, T.D. Mirsaev, K.R. Dorokhina. The role of vitamin D in maintaining oral health. Literature review. Actual problems in dentistry. 2020;16;4:25-34. (In Russ.)]. https://www.elibrary.ru/item.asp?id=44667773
25. Stecyuk O.U., Andreeva I.V. Sovremennye predstavleniya ob effektivnosti i prakticheskie podhody k primeneniyu probiotikov v klinicheskoy praktike:fokus na Lactobacillus rhamnosus GG i Bifidobacterium lactis Vb-12. KMAH. 2019;21;2:100-118. [O.U. Stetsyuk, I.V. Andreeva. Modern concepts of efficacy and practical approaches to the use of probiotics in clinical practice: focus on Lactobacillus rhamnosus GG and Bifidobacterium lactis Bb-12. KMAKH. 2019;21;2:100-118. (In Russ.)].
26. Haritonova L.A., Grigor'ev K.I., Borzakova S.N. Mikrobiota cheloveka: kak novaya nauchnaya paradigma menyaet medicinskuyu praktiku. Eksperimental'naya i klinicheskaya gastroenterologiya. 2019;161;1:55-63. [L.A. Kharitonova, K.I. Grigoriev, S.N. Borzakova. Human microbiota: how a new scientific paradigm is changing medical practice. Experimental and Clinical Gastroenterology. 2019;161;1:55-63. (In Russ.)].
27. Harper E., Zaharova I.N. Mikrobioticheskie narusheniya kak mezhdisciplinarnaya problema global'nogo urovnya. Medicinskiy sovet. 2019;2:53-60. [E. Harper, I.N. Zakharova. Microbiotic disorders as an interdisciplinary problem of the global level. Medical Council. 2019;2:53-60. (In Russ.)].
28. Yablokova E.A., Gorelov A.V. Probiotiki: sovremennyy instrument v rukah klinicista. Pediatriya (Pril. k zhurn. Consilium Medicum). 2018;4:59-62. [E.A. Yablokova, A.V. Gorelov. Probiotics: a modern tool in the hands of the clinician. Pediatrics (Appendix to the journal Consilium Medicum). 2018;4:59-62. (In Russ.)].
29. Allaker R.P., Stephen A.S. Use of Probioitics and Oral Health // Curr Oral Health Rep. - 2017;4:309-318.
30. Belstrom D., Paster B.J., Fiehn N.E. et al. Salivary bacterial fingerprints of established oral disease revealed by the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS) technique // J Oral Microbiol. - 2016;8:30170.
31. Bustamante M. et al. Probioitics as an Ajanct Therapy for the Treatment of Halitosis, Dental Caries and Periodontitis // Probioitics and Antimicrobial Proteins. - 2019. https://doi.org/10.1007/s12602-019-9521-4
32. Coqueiro A.Y. et al. Probiotic sapplementation in dental caries: is it possible to replace conventional treatment? // Nutrire. - 2018;43:16.
33. Czaplewski L., Bax R., Clokie M., Dawson M.,Fairhead H., Fischetti V.A. et al. Alternatives to antibiotics - a pipeline portfolio review // Lancet Infect Dis. - 2016;16(2):239-251.
34. Chervinets Y. et al. Adaptation and Probiotic Potencial of Lactobacilli, Isolated from the Oral Cavity and Intestines of Healthy People // Probioitics and Antimicrobial Proteins. - 2018;10.
35. Hanel A.N., Herzog H.M., James M.G., Cuadra G.A. Effects of Oral Commensal Streptococci on Porphyromonas gingivalis Invasion into Oral Epithelial Cells // Dent. J. - 2020;8:39.
36. Ohshima J., Wang Q., Fitzsimonds Z.R., Miller D.P., Sztukowska M.N., Jung Y.J., Hayashi M., Whiteley M., Lamont R.J. Streptococcus gordonii programs epithelial cells to resist ZEB2 induction by Porphyromonas gingivalis // Proc. Natl. Acad. Sci. USA. - 2019;116:8544-8553.
